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Antibiotic resistance 1

Antibiotic resistance genes 1

Antibiotic resistance genes (ARGs) 1

CRISPR/Cas9 1

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: with lactoferrin promoter mutations were obtained with an efficiency of 24% (7/29) by injecting the plasmidpX330, expressing a small guide RNA and human codon-optimized SpCas9, into fertilized eggs of mice.Plasmid integration and off-targeting of pX330 were not detected.These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: The purpose of this work was to construct the plasmid that could direct the synthesis of siRNA-like transcriptsof the target gene were chemically synthesized and annealed and were then ligated with pSUPER EGFP1 plasmidThen the plasmids were transfected into the cells at different ratios of plasmid to Lipofectamine.After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1124-5

Abstract:

Plasmid DNA/ARGs were less readily broken down

Keywords： Antibiotic resistance     Antibiotic resistance genes (ARGs)     Extracellular DNA/ARGs     Chlorination    

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Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 6, doi: 10.1007/s11783-020-1287-0

Abstract: Abstract • Sub-inhibitory levels of nC60 promote conjugative transfer of ARGs. • nC60 can induce ROS generation, oxidative stress and SOS response. • nC60 can increase cell membrane permeability and alter gene expression. • Results provide evidence of nC60 promoting antibiotic resistance dissemination. The spread and development of antibiotic resistance globally have led to severe public health problems. It has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes (ARGs). Nanofullerene (nC60) is a type of nanomaterial widely used around the world, and some studies have discovered both the biological toxicity and environmental toxicity of nC60. In this study, cellular and molecular biology techniques were employed to investigate the influences of nC60 at sub-minimum inhibitory concentrations (sub-MICs) on the conjugation of ARGs between the E. coli strains. Compared with the control group, nC60 significantly increased the conjugation rates of ARGs by 1.32‒10.82 folds within the concentration range of 7.03‒1800 mg/L. This study further explored the mechanism of this phenomenon, finding that sub-MICs of nC60 could induce the production of reactive oxygen species (ROS), trigger SOS-response and oxidative stress, affect the expression of outer membrane proteins (OMPs) genes, increase membrane permeability, and thus promote the occurrence of conjugation. This research enriches our understanding of the environmental toxicity of nC60, raises our risk awareness toward nC60, and may promote the more rational employment of nC60 materials.

Keywords： Nanofullerene     Sub-minimum inhibitory concentrations     Antibiotic resistance genes     Conjugation     Molecular biological techniques    

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Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 2, doi: 10.1007/s11783-017-0986-7

Abstract: In this work, transport of an indigenous resistance plasmid pK5 in porous media was investigated throughA similarity in transport behavior was also found between pK5 and an engineered plasmid pBR322 that hasinfluence of surfactants, a major group of constituents in soil solutions, was examined using an engineered plasmidCetyltrimethyl ammonium bromide (CTAB), a cationic surfactant, was found to significantly enhance plasmid

Keywords： Indigenous plasmid     Transport     Porous media     Surfactants    

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Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 287-291 doi: 10.1007/s11684-009-0050-5

Abstract: In order to explore the role of cyclin-dependent kinase 4 (cdk4) in neurodegenerative diseases, lentiviral-delivered RNA interference (RNAi) was used to silence the expression of the murine cdk4 gene . Three cdk4-shRNAs of mouse and a negative sequence were designed. After synthesis and annealing, double strand oligonucleotides were cloned into a linearized pSIH1-H1-copGFP shRNA vector. It was confirmed by polymerase chain reaction (PCR) and sequencing that three pairs of cdk4-shRNAs and a negative shRNA were correctly inserted into the pSIH1-H1-copGFP vector. The above recombinants were transfected by lipofectamine into BV-2 cells. The gene silencing efficacy rates of the 3 targets were compared by Western blotting. The cdk4-siRNA2 was the most effective in silencing cdk4. The optimized pSIH1-cdk4-siRNA2 and pSIH-negative-siRNA were co-transfected into 293T cells with the lentiviral packaging plasmids respectively. The culture supernatant was harvested and condensed at the 24th and 48thh after transfection. Interference efficiency of the lentivirus expressing cdk4-siRNA was determined by reverse transcriptase-PCR (RT-PCR) and Western blotting in BV-2 cells. Lentivector-mediated RNAi could efficiently down-regulate the expression of the murine cdk4 gene , which provides a potential tool for studying and treating cdk4-related diseases.

Keywords： cyclin-dependent kinase 4     RNA interference     plasmid     lentiviral vector    

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Title Author Date Type Operation

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Degradation of extracellular genomic, plasmid DNA and specific antibiotic resistance genes by chlorination

Menglu Zhang, Sheng Chen, Xin Yu, Peter Vikesland, Amy Pruden

Journal Article

Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance

Qingkun Ji, Caihong Zhang, Dan Li

Journal Article

Transport of antibiotic resistance plasmids in porous media and the influence of surfactants

Peipei Chen, Chaoqi Chen, Xiqing Li

Journal Article

Lentivector-mediated RNAi efficiently downregulates expression of murine cdk4 gene

Feng JIANG PhD , Xuezhen WANG PhD , Zheng XUE MD , Suming ZHANG PhD , Siyu FANG BM , Min ZHANG MD, PhD ,

Journal Article